APE1, also known as HAP1 or Ref1, acts as an AP lyase by hydrolyzing the phosphodiester backbone at the 5′ end of an apurinic (AP) site, generating a 1 base gap in the DNA duplex and leaving 3′-hydroxyl and 5′-deoxyribose phosphate termini.

Description:APE1, also known as HAP1 or Ref1, acts as an AP lyase by hydrolyzing the phosphodiester backbone at the 5\'-end of an apurinic (AP) site, generating a 1 base gap in the DNA duplex and leaving 3\'-hydroxyl and 5\'-deoxyribose phosphate termini. Evidence suggests that APE1 may exhibit weak DNA 3\'-diesterase, 3\' to 5\'-exonuclease and RNase H activities (1-4).Specific Activity: 2,000,000 U/mgApplication:- Single cell gel electrophoresis (Comet assay)- Alkaline elution- Alkaline unwinding- Modified nick translationSource:An E. coli strain which carries the cloned human APE1 gene.Supplied in:10 mM Tris-HCl50 mM NaCl1 mM Dithiothreitol0.1 mM EDTA50% GlycerolpH 8.0 @ 25°CSupplied with:10x Green Buffer10x Green Buffer:200 mM Tris-Acetate500 mM Potassium Acetate10 mM Dithiothreitol100 mM Magnesium AcetatepH 7.9 @ 25°CUnit Definition:One unit is defined as the amount of enzyme required to cleave 20 pmol of a 34-mer oligonucleotide duplex contain-ing a single AP site in 1 hour at 37°C.Recommended Storage Condition: -20ºCReference:1. Demple, B. et al. (1991) Proc. Natl. Acad. Sci. USA, 88, 11450-11454.2. Barzilay, G. et al. (1995) Nucl. Acids Res., 23, 1544-1550.3. Barzilay, G. et al. (1995) Nature Struc. Biol., 2, 451-468.4. Unpublished observations (See “Notes” Section)