For DNA sequencing high GC regions and rapid sequencing from nanogram amounts of DNA templates.

Description:A recombinant E. coli strain carrying the BST DNA Polymerase gene (large fragment). 

\"Bst.jpg\"\"Figure: MCLAB Bst DNA Polymerase (large fragment) shows higher strand -displacing polymerase activity than two other vendors\' products.

Application:- Sequencing through problematic secondary structuresSource:Bst DNA Polymerase (large fragment) is a portion of Bacillus stearothermophilus DNA Polymerase protein that contains 5´-> 3´ polymerase activity, but lacks 5\'->3\' and 3\'->5\' exonuclease activities.Specific Activity: 120,000 U/mgSupplied in:10 mM Tris-HCl50 mM KCl1.0 mM DTT0.1 mM EDTA0.1% Triton X-10050% GlycerolpH 7.5 @ 25°CSupplied with:10X PCR buffer II10x PCR Buffer ll: 200 mM Tris-HCl100 mM Ammonium Sulfate100 mM KCl20 mM MgSO41.0% Triton X-100pH 8.8 @ 25°CUnit Definition:1 unit is defined as the amount of polymerase required to convert 10 nmol of dNTPs into acid insoluble material in 30 minutes at 65°C.Recommended Storage Condition: -20°CReference:Kiefer, et al. Structure 15 January 1997. 5, 95-108.