BacteriophageT4Lysozymebreaksdownbacterialcellwalls.Theenzymeattacksthepeptidoglycansinthecellwallsofbacteriaandhydrolyzestheβ-1,4linkagesbetweenN-acetylmuramicacidandN-acetylglucosamine.SpecificactivityofT4lysozymeissignificantlygreaterthaneggwhitelysozymewhenassayedwithMicrococcuslysodeikticusandE.coli.
Description:
BacteriophageT4Lysozymebreaksdownbacterialcellwalls.Theenzymeattacksthepeptidoglycansinthecellwallsofbacteriaandhydrolyzestheß-1,4linkagesbetweenN-acetylmuramicacidandN-acetylglucosamine.SpecificactivityofT4lysozymeissignificantlygreaterthaneggwhitelysozymewhenassayedwithMicrococcuslysodeikticusandE.coli.
Application:
-Bacteriallysisfornucleicacidextraction
-Bacteriallysisforrecombinantproteinextraction
Source:
ArecombinantE.colistraincarryingtheclonedbacteriophageT4lysozymegene.
UnitDefinition:
OneunitofT4lysozymeproducesadecreaseinA350of0.008persecondat23°Cwitha0.5mg/mlsUSPensionoflyophilizedM.lysodeikticusin0.02Mphosphatebuffer(pH6.7).
RecommendedStorageCondition:-20°C
Reference:
Tsugita,A.,andInouye,M.PurificationofBacteriophageT4Lysozyme.TheJournalofBIOLOGicalChemistry,243,391-397.(1968) |
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Established in 1998, Molecular Cloning Laboratories (MCLAB) is a leader in providing genomic research consumables and services to the Life Sciences community.
MCLAB provides cost-effective consumables and reagents, focusing on first generation as well as next-generation sequencing and DNA fragment analysis. We manufacture a large selection of molecular biology related products, including antibodies, biochemical reagents, cloning kits, enzymes, PCR kits, protein gels & solutions and more.
With a team of over twenty experienced scientists and over six decades of experience, MCLAB is committed to offering first-rate services to researchers. We specialize in the field of genomics, with experts in DNA sequencing, fermentation, fragment analysis, molecular cloning, over-expression, protein purification, and protein synthesis.
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规模11-50 人
总部South San Francisco， CA
类型私人持股
创立1998
领域Next-generation Sequencing， DNA fragment analysis， Antibodies， Biochemical Reagents， Cloning Kits， Enzymes， PCR Kits， Protein Gels & Solutions， DNA Sequencing， Fermentation， Molecular Cloning， Protein Purification， Protein Synthesis
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