T4RNALigase2,alsoknownasT4Rnl2(gp24.1),hasbothintermolecularandintramolecularRNAstrandjoiningactivity.UnlikeT4RNALigase1,T4RNALigase2ismuchmoreactivejoiningnicksondoublestrandedRNAthanonjoiningtheendsofsinglestrandedRNA.Theenzymerequiresanadjacent5´phosphateand3´OHforligation.
Description:
T4RNALigase2,alsoknownasT4Rnl2(gp24.1),catalyzesbothinter-andintramolecularformationofphosphodiesterbondsbetween5'-phosphateand3'-hydroxylterminiofoligonucleotides,single-strandedRNAandDNA.UnlikeT4RNALigase1,T4RNALigase2ismuchmoreactiveonjoiningnicksondoublestrandedRNAthanonjoiningtheendsofsingle-strandedRNA.Theenzymerequiresanadjacent5'-phosphateand3'-OHforligation.Theenzymecanalsoligatethe3'-OHofRNAtothe5'-phosphateofDNAinadoublestrandedstructure. Source:
AnE.colistrainthatcarriestheT4RNALigase2gene(I.Schildkraut).
Application:
-LigatesanickindsRNA
-Ligatesthe3'-OHofRNAtothe5'-phosphateofDNAinadoublestrandedstructure
ReagentsSupplied:
T4Rnl2ReactionBuffer
ReactionConditions:
1xT4Rnl2ReactionBuffer
Incubateat37°C
10xT4RNALigase2ReactionBuffer:
500mMTris-HCl
20mMMgCl2
10mMDTT
4mMATP
pH7.5@25°C
UnitDefinition:
Oneunitisdefinedastheamountofenzymerequiredtoligate0.4µgofanequimolarmixofa23-merand17-merRNAsinatotalreactionvolumeof20µlin30minutesat37°C.
UnitAssayConditions:
1xT4Rnl2ReactionBufferand0.4µgofanequimolarmixofthe23-merand17-merRNAs.Afterincubationat37°Cfor30minutes,theligatedproductisdetectedona15%polyacrylamidegel. SpecificActivity:>40,000U/mg
StorageConditions:
10mMTris-HCl
50mMKCl
35mM(NH4)2SO4
0.1mMDTT
0.1mMEDTA
50%Glycerol
pH7.5@25°C
RecommendedStorageCondition:-20°C |
关于我们
Established in 1998, Molecular Cloning Laboratories (MCLAB) is a leader in providing genomic research consumables and services to the Life Sciences community.
MCLAB provides cost-effective consumables and reagents, focusing on first generation as well as next-generation sequencing and DNA fragment analysis. We manufacture a large selection of molecular biology related products, including antibodies, biochemical reagents, cloning kits, enzymes, PCR kits, protein gels & solutions and more.
With a team of over twenty experienced scientists and over six decades of experience, MCLAB is committed to offering first-rate services to researchers. We specialize in the field of genomics, with experts in DNA sequencing, fermentation, fragment analysis, molecular cloning, over-expression, protein purification, and protein synthesis.
MCLAB has offices in China and distributors all around the world, including Australia, Austria, Belgium, Canada, Czech Republic, Denmark, Germany, Hungary, Greece, India, Israel, Poland, New Zealand, Turkey, United Kingdom and Vietnam.
行业生物技术
规模11-50 人
总部South San Francisco， CA
类型私人持股
创立1998
领域Next-generation Sequencing， DNA fragment analysis， Antibodies， Biochemical Reagents， Cloning Kits， Enzymes， PCR Kits， Protein Gels & Solutions， DNA Sequencing， Fermentation， Molecular Cloning， Protein Purification， Protein Synthesis
地点
